6E9U

The crystal structure of bovine ultralong antibody BOV-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-23
Detector	RAYONIX MX-300
Wavelength(s)	0.978560
Spacegroup name	P 1 21 1
Unit cell lengths	50.261, 79.245, 62.164
Unit cell angles	90.00, 95.19, 90.00

Refinement procedure

Resolution	40.770 - 2.295
R-factor	0.2159
Rwork	0.214
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.554
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.790	2.420
High resolution limit [Å]	2.290	2.290
Rmerge	0.049	0.330
Number of reflections	21713
<I/σ(I)>	15.4
Completeness [%]	99.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	7% PEG 8000, 14% isopropanol, 0.1 M Tris-HCl pH 7.5