6E9O
E. coli D-galactonate:proton symporter mutant E133Q in the outward substrate-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.1158 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 217.070, 70.710, 107.160 |
Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
Resolution | 14.984 - 3.501 |
R-factor | 0.2862 |
Rwork | 0.285 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e9n |
RMSD bond length | 0.003 |
RMSD bond angle | 0.610 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.230 | 3.770 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmeas | 0.080 | 5.150 |
Number of reflections | 20394 | 4030 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 99.8 | |
Redundancy | 3.5 | |
CC(1/2) | 1.000 | 0.159 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 100mM glycine pH 9, 32% PEG 1000 |