6E9N
E. coli D-galactonate:proton symporter in the inward open form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.700, 107.630, 103.210 |
| Unit cell angles | 90.00, 108.32, 90.00 |
Refinement procedure
| Resolution | 48.277 - 2.915 |
| R-factor | 0.2461 |
| Rwork | 0.243 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pw4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 24356 | 512 |
| <I/σ(I)> | 6.4 | 0.6 |
| Completeness [%] | 70.0 | |
| Redundancy | 1.9 | 1.3 |
| CC(1/2) | 0.750 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.35 | 277 | 100mM sodium acetate pH 5.35, 39% PEG 400 |
