6E9K

The crystal structure of bovine ultralong antibody BOV-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-13
Detector	RAYONIX MX-300
Wavelength(s)	0.978560
Spacegroup name	P 1 21 1
Unit cell lengths	45.018, 70.795, 73.857
Unit cell angles	90.00, 107.07, 90.00

Refinement procedure

Resolution	43.035 - 2.193
R-factor	0.2066
Rwork	0.204
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.035	2.310
High resolution limit [Å]	2.190	2.190
Rmerge	0.037	0.428
Number of reflections	21489
<I/σ(I)>	13.5
Completeness [%]	94.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	25% PEG 4000, 6% tacsimate, 0.1 M MES pH 6.0