6E9H

The crystal structure of bovine ultralong antibody BOV-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-01
Detector	RAYONIX MX-300
Wavelength(s)	0.978720
Spacegroup name	P 21 21 21
Unit cell lengths	51.247, 71.219, 119.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.839 - 2.000
R-factor	0.2189
Rwork	0.217
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.120	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.048	0.224
Number of reflections	30426
<I/σ(I)>	18.6
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	30% PEG 1000, 0.1 M sodium malonate pH 8.0, 0.1M Tris-HCl pH 8.0