6E9G
The crystal structure of bovine ultralong antibody BOV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-03 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.978560 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.366, 71.926, 88.004 |
| Unit cell angles | 90.00, 96.56, 90.00 |
Refinement procedure
| Resolution | 47.590 - 2.904 |
| R-factor | 0.2227 |
| Rwork | 0.220 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.581 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.059 | 0.230 |
| Number of reflections | 11347 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291.15 | 10% PEG 200, 0.1 M Bis-Tris propane pH 9.0-9.5, 17% PEG 8000 |
