6E9C
Selenomethionine Derivative Structure of A Bacterial Homolog to Human Lysosomal Transporter, Spinster
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-06-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.590, 87.330, 122.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.978 - 3.200 |
R-factor | 0.2772 |
Rwork | 0.277 |
R-free | 0.28430 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.163 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 29749 | |
<I/σ(I)> | 10.27 | |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 32.5(v/v)% PEG 400, 100 mM Tris-HCl |