6E95

Chimeric structure of Saccharomyces cerevisiae GCN4 leucine zipper fused to Staphylococcus aureus AgrC cytoplasmic histidine kinase module (dataset isotropically truncated by HKL2000)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 2 21 21
Unit cell lengths	63.141, 83.375, 146.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.810 - 2.250
R-factor	0.22168
Rwork	0.219
R-free	0.27465
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.534
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.290
High resolution limit [Å]	2.250	2.250
Rmeas	0.083	1.043
Rpim	0.035	0.422
Number of reflections	37648	1824
<I/σ(I)>	13.4	2.2
Completeness [%]	100.0	99.9
Redundancy	6.5	5.9
CC(1/2)	0.930	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M Tris pH 8.0 and 2.8 M NaCl