6E8Z

Binary complex of Human glycerol 3-phosphate dehydrogenase with NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	63.580, 78.780, 69.179
Unit cell angles	90.00, 104.32, 90.00

Refinement procedure

Resolution	33.960 - 2.100
R-factor	0.1717
Rwork	0.169
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x0x
RMSD bond length	0.012
RMSD bond angle	1.160
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.960	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.047	0.195
Rmeas	0.056	0.234
Number of reflections	37384	3740
<I/σ(I)>	13.5
Completeness [%]	96.6	98.1
Redundancy	3.2	3.2
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287	200 mM potassium chloride, 23% PEG 20000, 0.1 M bis-tris propane pH 7.0, 10 mM NAD; 10 mg/mL