6E8Y

Unliganded Human Glycerol 3-Phosphate Dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	63.370, 76.160, 64.390
Unit cell angles	90.00, 96.03, 90.00

Refinement procedure

Resolution	47.480 - 1.850
R-factor	0.1723
Rwork	0.170
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e8z
RMSD bond length	0.011
RMSD bond angle	1.048
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.074	0.314
Rmeas	0.089	0.385
Number of reflections	51748	5043
<I/σ(I)>	8
Completeness [%]	99.4	97.6
Redundancy	3.2	3
CC(1/2)	0.995	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287	175 mM potassium chloride, 23% PEG 20000, 0.1M bis-tris propane pH 7.0; 10 mg/mL