6E8Y
Unliganded Human Glycerol 3-Phosphate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.370, 76.160, 64.390 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 47.480 - 1.850 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e8z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.048 |
| Data reduction software | MOSFLM (7.2.1) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.480 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.074 | 0.314 |
| Rmeas | 0.089 | 0.385 |
| Number of reflections | 51748 | 5043 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 3.2 | 3 |
| CC(1/2) | 0.995 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 175 mM potassium chloride, 23% PEG 20000, 0.1M bis-tris propane pH 7.0; 10 mg/mL |
