6E8V

The crystal structure of bovine ultralong antibody BOV-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-21
Detector	RAYONIX MX300HE
Wavelength(s)	0.97856
Spacegroup name	P 42 21 2
Unit cell lengths	308.219, 308.219, 133.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.203 - 3.790
R-factor	0.2212
Rwork	0.218
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.654
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.840	3.232
High resolution limit [Å]	3.196	3.196
Rmerge	0.216	1.440
Number of reflections	89101
<I/σ(I)>	8.5
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	14-18% PEG 3350, 0.1 M Citric acid pH 4.0-45., 0.1 M sodium citrate tribasic