6E8U
Structure of the Mango-III (A10U) aptamer bound to TO1-Biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 53.270, 53.270, 188.036 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.590 - 1.550 |
| R-factor | 0.1794 |
| Rwork | 0.177 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e8s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.123 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.590 | 1.610 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.050 | 1.000 |
| Number of reflections | 20010 | 1951 |
| <I/σ(I)> | 20.9 | 1.5 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 10.2 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 0.1 M NaCacodylate pH 7.1, PEG3350 16%, 0.25 M Magnesium Acetate |
