6E8R

Structure of the SNX32 PX domain in complex with Chlamydial protein IncE in space group I121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	I 1 2 1
Unit cell lengths	90.877, 46.004, 134.207
Unit cell angles	90.00, 105.59, 90.00

Refinement procedure

Resolution	43.767 - 2.268
R-factor	0.189
Rwork	0.186
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tgi
RMSD bond length	0.017
RMSD bond angle	1.312
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.767	2.340
High resolution limit [Å]	2.268	2.268
Rmerge	0.151	0.894
Rmeas	0.176	1.046
Rpim	0.063	0.540
Number of reflections	25107	2222
<I/σ(I)>	10.7	2.5
Completeness [%]	99.7	97
Redundancy	7.5	7.2
CC(1/2)	0.996	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	20% PEG 8000, 0.1 M TRIS (pH 8.0), 0.01 M MgCl2