6E8M
Legionella Longbeachae LeSH (Llo2327) bound to the human DnaJ-A1 pTyr381 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 114 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.900, 64.710, 67.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.210 - 1.610 |
| R-factor | 0.1992 |
| Rwork | 0.199 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e8h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.843 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.920 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.052 | 0.503 |
| Number of reflections | 22071 | 3192 |
| <I/σ(I)> | 24.9 | 4.1 |
| Completeness [%] | 90.3 | 91.2 |
| Redundancy | 11 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 296 | PEG3000, malic acid, Tris-HCl |
