6E8K
Legionella Longbeachae LeSH (Llo2327) bound to the human interleukin-2 receptor beta pTyr387 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 114 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.450, 64.240, 67.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.920 - 1.710 |
R-factor | 0.1894 |
Rwork | 0.188 |
R-free | 0.21120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e8h |
RMSD bond length | 0.006 |
RMSD bond angle | 0.812 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.700 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.052 | 0.491 |
Number of reflections | 19223 | 2448 |
<I/σ(I)> | 12.6 | 2.4 |
Completeness [%] | 91.3 | 86.7 |
Redundancy | 5.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 296 | PEG3000, sodium malonate |