6E8H
Legionella Longbeachae LeSH (Llo2327)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 114 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.260, 66.510, 68.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.680 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.24750 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.052 | 0.284 |
| Number of reflections | 22693 | 2832 |
| <I/σ(I)> | 68.2 | 16.9 |
| Completeness [%] | 97.5 | 85 |
| Redundancy | 55.7 | 50.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 278 | Tris, PEG3000, sodium acetate |
