6E8F
Crystal Structure of Human Protocadherin-15 EC3-5 CD2-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 95.735, 95.906, 258.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 2.990 |
| R-factor | 0.17583 |
| Rwork | 0.174 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t4m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Rmerge | 0.208 | 0.814 |
| Number of reflections | 48387 | 2333 |
| <I/σ(I)> | 9.31 | 2.44 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 10.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 40% MPD, 0.2 M Lithium Chloride, 0.01 M Adenosine triphosphate disodium |
