6E8D
Crystal structure of the Bacillus subtilis sliding clamp-MutL complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-27 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97951 |
Spacegroup name | P 1 |
Unit cell lengths | 59.020, 83.890, 128.260 |
Unit cell angles | 80.30, 83.57, 89.96 |
Refinement procedure
Resolution | 48.255 - 2.340 |
R-factor | 0.2164 |
Rwork | 0.216 |
R-free | 0.25020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.732 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.255 | 2.420 |
High resolution limit [Å] | 2.340 | 2.340 |
Number of reflections | 98638 | |
<I/σ(I)> | 13.4 | |
Completeness [%] | 97.1 | |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM HEPES pH 7.5, 25 % PEG 3,350, 200 mM ammonium sulfate. |