6E8C

Crystal structure of the double homeodomain of DUX4 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9668
Spacegroup name	C 2 2 21
Unit cell lengths	67.190, 73.140, 108.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.024 - 2.120
R-factor	0.201
Rwork	0.198
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lnq
RMSD bond length	0.011
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX ((dev_3126: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.180	2.200
High resolution limit [Å]	2.120	2.120
Rmerge	0.054	1.370
Rmeas	0.066
Rpim	0.037
Number of reflections	15112
<I/σ(I)>	14
Completeness [%]	93.9	83
Redundancy	3	13.6
CC(1/2)	0.996	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 3350 0.1M Bis-TRIS pH 6.5 5% glycerol