6E7E
High resolution crystal structure of IncA soluble domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.323, 48.822, 41.738 |
| Unit cell angles | 90.00, 103.91, 90.00 |
Refinement procedure
| Resolution | 14.960 - 1.120 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.16900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0241) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.160 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Number of reflections | 52606 | 2307 |
| <I/σ(I)> | 47.9 | 4.7 |
| Completeness [%] | 91.3 | 55 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.2 M sodium acetate trihydrate, pH 8.0, 20% w/v PEG3350 |
