6E75

Structure of Human Transthyretin Asp38Ala Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	43.190, 85.740, 63.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 1.500
R-factor	0.186
Rwork	0.184
R-free	0.20700
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.900	19.900	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.037	0.024	0.499
Rmeas	0.040	0.026	0.551
Number of reflections	38583	480	2794
<I/σ(I)>	21.83	49.89	3.21
Completeness [%]	99.5	93.9	98.6
Redundancy	5.748	5.279	5.555
CC(1/2)	0.999	0.999	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	30% (v/v) PEG 400, 100 mM Sodium acetate, 200 mM Calcium acetate