6E71
Structure of Human Transthyretin Val30Met/Thr119Met Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.140, 86.540, 63.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.270 - 1.500 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.21200 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.270 | 43.270 | 1.540 |
High resolution limit [Å] | 1.500 | 6.710 | 1.500 |
Rmerge | 0.047 | 0.020 | 0.907 |
Rmeas | 0.056 | 0.025 | 1.083 |
Number of reflections | 38230 | 488 | 2820 |
<I/σ(I)> | 13.95 | 39.98 | 1.54 |
Completeness [%] | 98.0 | 94.4 | 98.7 |
Redundancy | 3.249 | 2.906 | 3.214 |
CC(1/2) | 0.999 | 0.999 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M Lithium Sulfate, 0.1M Tris, 30% (w/v) PEG 4000 |