6E6L
Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:K40L:T51F:Y60A mutant of human cellular retinol binding protein II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-26 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.765, 60.554, 36.573 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.909 - 2.080 |
R-factor | 0.2029 |
Rwork | 0.198 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.007 |
RMSD bond angle | 0.814 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.909 | 2.154 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.091 | 1.200 |
Rmeas | 0.094 | |
Rpim | 0.336 | |
Number of reflections | 8971 | 442 |
<I/σ(I)> | 34.2 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.2 | |
CC(1/2) | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, sodium acetate, Ammonium acetate |