6E6H

NRAS G13D bound to GppNHp (N13GNP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	173.15
Detector technology	IMAGE PLATE
Collection date	2014-07-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	59.553, 39.565, 65.016
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	32.500 - 1.990
Rwork	0.179
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.098	0.058	0.567
Rmeas	0.112	0.066	0.668
Rpim	0.051	0.029	0.343
Total number of observations	42385
Number of reflections	9752	524	411
<I/σ(I)>	8
Completeness [%]	97.0	98.9	86.3
Redundancy	4.3	4.6	3.4
CC(1/2)		0.996	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	9% 2-propanol, 72 mM HEPES, 20% PEG 4000, 15% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, at pH 7.5), Crystals were grown in 1uL by 1uL drops of mother liquor to protein (12.2 mg/mL) No cryoprotectant used for diffraction