6E65

Crystal structure of malaria transmission-blocking antibody TB31F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.999977
Spacegroup name	P 21 21 21
Unit cell lengths	55.680, 70.720, 115.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.698 - 1.500
R-factor	0.1752
Rwork	0.175
R-free	0.18700
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	1.600
High resolution limit [Å]	1.500	1.500
Number of reflections	73693	12770
<I/σ(I)>	15.9	1.8
Completeness [%]	100.0	100
Redundancy	13	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES, pH 7.5, 10% (v/v) 2-propanol, 20% (w/v) PEG 4000