6E64

Crystal structure of malaria transmission-blocking antibody 85RF45.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979490
Spacegroup name	P 21 21 21
Unit cell lengths	71.879, 86.522, 139.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.372 - 3.150
R-factor	0.2477
Rwork	0.246
R-free	0.27920
RMSD bond length	0.004
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	3.250
High resolution limit [Å]	3.150	3.150
Number of reflections	15740	1394
<I/σ(I)>	7.2	1.5
Completeness [%]	99.8	99.9
Redundancy	12.9	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium thiocyanate, pH 6.9, 20% (w/v) PEG 3350