6E5X

Crystal structure of Ebola virus VP30 C-terminus/RBBP6 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-25
Detector	NOIR-1
Wavelength(s)	1.00003
Spacegroup name	P 43 21 2
Unit cell lengths	45.600, 45.600, 158.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.830 - 1.500
R-factor	0.19109
Rwork	0.189
R-free	0.23021
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vap
RMSD bond length	0.002
RMSD bond angle	0.659
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.830	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.050	0.020
Rmeas	0.050	0.020
Number of reflections	27964	1327
<I/σ(I)>	30.4	0.7
Completeness [%]	100.0	99.3
Redundancy	16.7	10.5
CC(1/2)	0.300	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 18% w/v PEG8000