6E5F
Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-06-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | I 4 |
Unit cell lengths | 80.004, 80.004, 59.239 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.570 - 1.370 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.20350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e5d |
RMSD bond length | 0.004 |
RMSD bond angle | 0.764 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc1_3177) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.571 | 1.380 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.058 | 1.264 |
Rmeas | 0.062 | 1.510 |
Rpim | 0.021 | 0.806 |
Number of reflections | 40174 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 98.4 | 70.6 |
Redundancy | 8.4 | 3.2 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M sodium citrate, pH 6.5, 1.9 M ammonium sulfate |