6E52
Chimeric structure of Saccharomyces cerevisiae GCN4 leucine zipper fused to Staphylococcus aureus AgrC cytoplasmic histidine kinase module (dataset anisotropically truncated by STARANISO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 63.141, 83.375, 146.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.930 |
R-factor | 0.22081 |
Rwork | 0.218 |
R-free | 0.26807 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.521 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.760 | 1.980 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmeas | 0.087 | 1.405 |
Rpim | 0.035 | 0.655 |
Number of reflections | 36936 | 312 |
<I/σ(I)> | 11.9 | 1.2 |
Completeness [%] | 61.6 | 7.3 |
Redundancy | 6.4 | 4.4 |
CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris pH 8.0 and 2.8 M NaCl |