6E51
Crystal structure of the apo domain-swapped dimer Q108K:K40L:T51W mutant of human cellular retinol binding protein II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.077, 60.828, 36.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.850 - 2.256 |
| R-factor | 0.216 |
| Rwork | 0.208 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.850 | 2.300 |
| High resolution limit [Å] | 2.256 | 2.260 |
| Rmerge | 0.120 | 1.200 |
| Rmeas | 0.128 | |
| Number of reflections | 7191 | 10581 |
| <I/σ(I)> | 28.5 | 2.41 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, ammonium acetate, Sodium acetate |
