6E4X

Human antibody S5V2-29 in complex with influenza hemagglutinin A/Texas/50/2012 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	115.740, 163.480, 136.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.370 - 2.250
R-factor	0.2036
Rwork	0.203
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.370	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.073
Number of reflections	61532	31682
<I/σ(I)>	14.81
Completeness [%]	97.7	99.05
Redundancy	5.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	100 mM magnesium sulfate, 100 mM PIPES, pH 6.0, 30% PEG400