6E4W

Structure of AMPK bound to activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	123.970, 123.970, 402.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.580 - 3.350
R-factor	0.21
Rwork	0.209
R-free	0.24400
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	autoXDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.580	3.470
High resolution limit [Å]	3.350	3.350
Rmerge	0.122	0.689
Number of reflections	27326
<I/σ(I)>	16.2
Completeness [%]	100.0
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	750 MM AMMONIUM SULFATE, 500 MM LITHIUM SULFATE, 100 MM TRISODIUM CITRATE, 1% ETHYLENE GLYCOL