6E4T
Structure of AMPK bound to activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 123.610, 123.610, 402.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.010 - 3.400 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.24500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | autoXDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.010 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.137 | 0.659 |
| Number of reflections | 25978 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 750 MM AMMONIUM SULFATE, 500 MM LITHIUM SULFATE, 100 MM TRISODIUM CITRATE, 1% ETHYLENE GLYCOL |
