6E4O

Structure of apo T. brucei RRM: P4(1)2(1)2 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	72.100, 72.100, 118.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.676 - 1.800
R-factor	0.1674
Rwork	0.165
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e4n
RMSD bond length	0.006
RMSD bond angle	0.890
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.676	1.970
High resolution limit [Å]	1.800	1.800
Rpim	0.035	0.208
Number of reflections	28420
<I/σ(I)>	12.5	2.3
Completeness [%]	96.0	80.3
Redundancy	2.6	1.4
CC(1/2)	0.997	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.2-1.4 M trisodium citrate, HEPES buffer