Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.346, 41.795, 146.968 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.900 |
| R-factor | 0.20387 |
| Rwork | 0.201 |
| R-free | 0.25264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.960 |
| Number of reflections | 21636 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 92.9 | 95.6 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 1.000 | 0.210 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 298 | 100 mM MIB (Qiagen), pH 9.0, 25% PEG1500 |
