6E3K

Interferon gamma signalling complex with IFNGR1 and IFNGR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	78.284, 151.464, 211.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.188 - 3.250
R-factor	0.1923
Rwork	0.190
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fg9 5eh1
RMSD bond length	0.003
RMSD bond angle	0.589
Data reduction software	XDS (June 1, 2017)
Data scaling software	Aimless (0.5.3.2)
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.14_3211)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	3.380
High resolution limit [Å]	3.250	3.250
Rmerge	0.138	1.454
Rmeas	0.149	1.573
Rpim	0.058	0.594
Number of reflections	40393	4469
<I/σ(I)>	11.1	1.2
Completeness [%]	99.9	99.9
Redundancy	6.6	6.8
CC(1/2)	0.994	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	100 mM Bis Tris Propane pH 7.0, 2 M sodium formate