6E3D
Atomic structure of Mycobacterium tuberculosis DppA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.739, 69.092, 62.363 |
| Unit cell angles | 90.00, 106.52, 90.00 |
Refinement procedure
| Resolution | 38.007 - 1.271 |
| R-factor | 0.1328 |
| Rwork | 0.132 |
| R-free | 0.16530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b7h |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rpim | 0.003 | 0.029 |
| Number of reflections | 102717 | |
| <I/σ(I)> | 37.8 | |
| Completeness [%] | 91.1 | 51.3 |
| Redundancy | 6.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.2 M Sodium Malonate pH 6.0 and 22% (w/v) PEG3350 |
