6E3A

tRNA 2'-phosphotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.20510
Spacegroup name	P 61 2 2
Unit cell lengths	53.367, 53.367, 308.903
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.217 - 1.400
R-factor	0.1625
Rwork	0.162
R-free	0.18570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wfx
RMSD bond length	0.015
RMSD bond angle	1.467
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rpim	0.016	0.252
Number of reflections	53083	2505
<I/σ(I)>	24.1
Completeness [%]	99.8	97.8
Redundancy	17.5	10.9
CC(1/2)	0.995	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MES pH 6.0, 10 %MPD