6E33
Crystal Structure of Pho7-DNA complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.2827, 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.827, 40.682, 58.237 |
| Unit cell angles | 90.00, 105.84, 90.00 |
Refinement procedure
| Resolution | 43.131 - 1.705 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.21520 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.040 | |
| Number of reflections | 24169 | 1086 |
| <I/σ(I)> | 24.1 | 2.5 |
| Completeness [%] | 96.1 | 87.4 |
| Redundancy | 5.5 | 3.6 |
| CC(1/2) | 0.932 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Tris-HCl pH 7.0, 0.2M NaOAc, 32.5% PEG 4000 |
