6E31
Crystal structure of RIAM in an autoinhibited configuration.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.495, 83.956, 45.200 |
Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.21277 |
Rwork | 0.211 |
R-free | 0.23985 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tca |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 19178 | 942 |
<I/σ(I)> | 32.05 | 2.04 |
Completeness [%] | 98.0 | 97.7 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Magnesium acetate, 6% PEG 3350 |