6E31
Crystal structure of RIAM in an autoinhibited configuration.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.495, 83.956, 45.200 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.21277 |
| Rwork | 0.211 |
| R-free | 0.23985 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tca |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 19178 | 942 |
| <I/σ(I)> | 32.05 | 2.04 |
| Completeness [%] | 98.0 | 97.7 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Magnesium acetate, 6% PEG 3350 |
