6E2R

Mechanism of cellular recognition by PCV2

This is a large structure.

EM specimen

Vitrificationfalse
Stainingfalse
InstrumentFEI VITROBOT MARK IV

EM Data Collection

MicroscopeFEI TITAN
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorGATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)2.8
Number of Particle54409
Software,Leginon,Gctf,Coot,RELION,cryoSPARC,PHENIX
SymmetryI
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION