6E24

Ternary structure of c-Myc-TBP-TAF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97887
Spacegroup name	P 43
Unit cell lengths	73.136, 73.136, 78.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.500 - 3.001
R-factor	0.186
Rwork	0.184
R-free	0.22420
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	3.108
High resolution limit [Å]	3.001	3.001
Number of reflections	8361
<I/σ(I)>	18.91
Completeness [%]	100.0
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	16% W/V PEG3350 0.05M CITRIC ACID 0.05M BIS-TRIS PROPANE pH5.0