6E20
Crystal structure of the Dario rerio galectin-1-L2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-22 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 61 |
| Unit cell lengths | 40.438, 40.438, 303.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.810 - 2.000 |
| R-factor | 0.17726 |
| Rwork | 0.174 |
| R-free | 0.23405 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gan |
| RMSD bond length | 0.113 |
| RMSD bond angle | 1.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.218 |
| Number of reflections | 18718 | 1262 |
| <I/σ(I)> | 33.5 | 6.5 |
| Completeness [%] | 99.2 | 97.3 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M Magnesium Chloride Hexahydrate, 30% (v/v) PolyethG 400 |
