6E1X
Crystal structure of product-bound complex of spermidine/spermine N-acetyltransferase SpeG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 185.979, 186.502, 73.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.350 |
| R-factor | 0.12473 |
| Rwork | 0.123 |
| R-free | 0.15120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mi4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.721 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.053 | 0.653 |
| Number of reflections | 276661 | 13600 |
| <I/σ(I)> | 33.95 | 3.13 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.01 M magnesium chloride, 0.1 M Tris, 2% isopropanol |
