6E1R

Crystal structure of the Acinetobacter phage vB_ApiP_P1 tailspike protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	81.123, 90.015, 508.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.115 - 2.693
R-factor	0.2015
Rwork	0.200
R-free	0.23750
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	HKL2Map
Refinement software	PHENIX ((dev_3092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.690	2.690
Rmerge	0.160	0.879
Rmeas	0.173	0.950
Number of reflections	197955	31287
<I/σ(I)>	9.93	2.12
Completeness [%]	99.4	97.7
Redundancy	7.1	6.9
CC(1/2)	0.998	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	100mM sodium Citrate pH 5.5, 2.8M NaCl, 10mM KSCN