6E1J
Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.749, 90.277, 93.975 |
| Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
| Resolution | 47.745 - 2.096 |
| R-factor | 0.1524 |
| Rwork | 0.150 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rmj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.894 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 79220 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 92.4 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285.15 | 100 mM imidazole, 100 mM MES monohydrate (pH 6.5), 30 mM manganese chloride tetrahydrate, 30 mM calcium chloride dihydrate, 20% (v/v) ethylene glycol, and 10% (w/v) PEG-8000 |
