6E1C
Crystal structure of a MauG-like protein associated with microbial copper homeostasis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2017-04-22 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.722003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.557, 85.757, 133.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.879 - 2.617 |
R-factor | 0.2046 |
Rwork | 0.198 |
R-free | 0.27810 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.664 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.880 | 2.690 |
High resolution limit [Å] | 2.617 | 2.617 |
Rmerge | 0.148 | 0.846 |
Rmeas | 0.152 | 0.871 |
Rpim | 0.035 | 0.202 |
Number of reflections | 24860 | |
<I/σ(I)> | 21.26 | |
Completeness [%] | 97.6 | |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | PPG 400, Bis-Tris |