6E1C

Crystal structure of a MauG-like protein associated with microbial copper homeostasis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.722003
Spacegroup name	P 21 21 21
Unit cell lengths	71.557, 85.757, 133.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.879 - 2.617
R-factor	0.2046
Rwork	0.198
R-free	0.27810
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.664
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.880	2.690
High resolution limit [Å]	2.617	2.617
Rmerge	0.148	0.846
Rmeas	0.152	0.871
Rpim	0.035	0.202
Number of reflections	24860
<I/σ(I)>	21.26
Completeness [%]	97.6
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	PPG 400, Bis-Tris