6E1C
Crystal structure of a MauG-like protein associated with microbial copper homeostasis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-04-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.722003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.557, 85.757, 133.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.879 - 2.617 |
| R-factor | 0.2046 |
| Rwork | 0.198 |
| R-free | 0.27810 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.880 | 2.690 |
| High resolution limit [Å] | 2.617 | 2.617 |
| Rmerge | 0.148 | 0.846 |
| Rmeas | 0.152 | 0.871 |
| Rpim | 0.035 | 0.202 |
| Number of reflections | 24860 | |
| <I/σ(I)> | 21.26 | |
| Completeness [%] | 97.6 | |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | PPG 400, Bis-Tris |
