6E18

Crystal structure of Chlamydomonas reinhardtii HAP2 ectodomain provides structural insights of functional loops in green algae.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9786
Spacegroup name	P 63 2 2
Unit cell lengths	125.620, 125.620, 367.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.990 - 2.600
R-factor	0.215
Rwork	0.214
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mf1
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.130	40.130	2.670
High resolution limit [Å]	2.600	11.620	2.600
Rmerge	0.059	0.055	0.213
Rmeas	0.406	0.057	21.903
Rpim	0.091	0.014	4.863
Number of reflections	53971	739	3919
<I/σ(I)>	8.2
Completeness [%]	99.9	97.5	99.6
Redundancy	19.6	15.6	19.9
CC(1/2)	0.997	0.999	0.244

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.2M LiSO4, 0.1M Tris-HCl pH 8.5 and 25% w/v PEG 5000 MME