6E16
Ternary structure of c-Myc-TBP-TAF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-03-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | P 43 |
Unit cell lengths | 72.816, 72.816, 78.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.408 - 2.400 |
R-factor | 0.1785 |
Rwork | 0.177 |
R-free | 0.21990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 40BA |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.408 | 2.510 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 16091 | |
<I/σ(I)> | 13.75 | |
Completeness [%] | 99.4 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 291 | 20% (w/w) PEG8000, 0.2M ammonium sulfare, and 0.1M HEPES pH7.5 |