6E0X
A131E mutant of cyt P460 of Nitrosomonas sp. AL212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.152, 80.356, 120.033 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 47.924 - 1.974 |
| R-factor | 0.2077 |
| Rwork | 0.206 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6amg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_3126)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.630 | 2.005 |
| High resolution limit [Å] | 1.970 | 1.974 |
| Rmerge | 0.053 | 1.110 |
| Number of reflections | 62608 | 62608 |
| <I/σ(I)> | 32 | |
| Completeness [%] | 96.1 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.2 M sodium chloride, 0.1 M sodium acetate, PEG 6000 |
